Rapid and Visual Detection of Coxiella burnetii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips

Table 1

Primers and probe for PCR, RPA, and qPCR.

Usages	Primer or probe	Nucleotide sequences (5′-3′)
PCR	CbF1	AAGGATCCAATTAACCGTTGTAGTT
	CbR1042	CGGAATTCTCACTCTTTCCTATGTT
RPA	CbF310	TCCTTGTCGGGTAAAAAATTGCCCCGCTAA
	CbF340	ACTGTAAAGTTTAGTGATAAAGTCAGCTCA
	CbF370	TATCGGGGGAACCCTCCTGCTTTTTAGCAA
	CbF399	AAGGGCAATCCCGAGGGAAGTCTTAAATGA
	CbR484	Biotin-AGTCAGCGTATTGCACACAAATGCGTGCCT
	CbR507	Biotin-TGAGTATAAACCCAAGGGCAAGAAGTCAGC
	CbR533	Biotin-TAAATTCTCCATAGTCACTTACTTCTTGAG
	CbR564	Biotin-CATACCATGGCTCTAAATGTAAATACATAA
	Cbprobe408	FAM-CCCGAGGGAAGTCTTAAATGACCCCGTAAC-[THF]-ACTGATCCGAAAGGT-PO4
RT-qPCR [22]	CbF	CGGCTGAATTTAAGCGATTTATTTTT
	CbR	CGTAACCACACACGCATCTCA
	TaqMan-MGB probe	TGCAATGGGTTCGG