Geminin Orchestrates Somite Formation by Regulating Fgf8 and Notch Signaling

Figure 2

Fgf8 and RA signaling mediate geminin to regulate proper somite space. (a-d) When compared with that in control (a, c) fgf8 expression was upregulated in geminin and p53 double morphants (b, 86%, n=28), but the expression of raldh2 was downregulated in geminin morphants (d, 81%, n=26). The embryos dominantly expressed GFP and Fgf8 in the left side (e, n=5); overexpression of Fgf8 in the left side of the embryos gave rise to narrow somite (e, left side, 100%, n=5). In the embryos coinjected with geminin MO and fgf8 MO (f, right side, 77%, n=22), the somite size is close to that in control embryos. Bar, 100μM.