Research Article
Geminin Orchestrates Somite Formation by Regulating Fgf8 and Notch Signaling
Figure 5
Brg1 helps geminin to bind the intron of Mib1 during regulating Mib1 transcription. (a, b) The ChIP experiments and the sequential qPCR showed that the quantity of intron 3 of Mib1 bound to geminin was decreased in Brg1 morphants (a). The luciferase report analysis showed that down- and/or upregulating the activity of Brg1 decreased or increased the luciferase activity, respectively (b). (c-j) Compared with that in control morphants (c, d, 86.7% and 84.4%, n=30 and 32), the expressions of Mib1 and her4 were downregulated in Brg1 morphants (d, h, 73.7% and 77.5%, n=38 and 40) and geminin morphants (e, i, 83.3% and 84.6%, n=36 and 39), respectively. Meanwhile in Brg1 and geminin double morphants (f, j, 84.4% and 86.7%, n=32 and 30) Mib1 and her4 were downregulated strongly. Bar, 100μM.
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