Purification and Characterization of Antibodies in Single-Chain Format against the E6 Oncoprotein of Human Papillomavirus Type 16

Figure 2

Characterization of the scFvs binding to 16E6. (a) Specificity of I7 and I7nuc scFvs binding to recombinant 16E6 in ELISA. Three experiments were performed and similar results were obtained. Data represent the mean ± SD of samples in quadruplicate from a representative experiment. < 0,01; > 0,05 is considered not significant (ns). (b) Sensitivity of scFv binding to recombinant 16E6 in slot blotting analysis. Serial dilutions of the recombinant 16E6 protein or BSA as a negative control were incubated with the indicated concentrations of scFvI7. Anti-16E6 polyclonal antibody was used as a positive control in (a) and (b).

(a)

(b)