Purification and Characterization of Antibodies in Single-Chain Format against the E6 Oncoprotein of Human Papillomavirus Type 16

Figure 3

Stability of the anti-16E6 scFv proteins. (a) Residual E6-binding activity of the purified scFvI7 (filled circle) and scFvI7nuc (empty circle) was analyzed in ELISA after incubation at 37°C for different time intervals. Data are expressed as a percentage of the antibody binding without incubation at 37°C and represent the mean ± standard deviation (SD) of three independent experiments. . (b) Kinetics of scFv protein degradation at 37°C were analyzed in WB.

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