Purification and Characterization of Antibodies in Single-Chain Format against the E6 Oncoprotein of Human Papillomavirus Type 16

Figure 4

Detection of endogenous 16E6 protein by scFvI7nuc in confocal microscopy. SiHa cells were stained with purified scFvI7nuc (green) and anti-16E6 polyclonal IgG (polyAb, red) used alone (a) or in combination (b). Cell nuclei were counterstained using RedDot™2 dye and are displayed in blue. Yellow staining in the "merge" image indicates detection of nuclear E6 by both antibodies. Samples were analyzed using confocal microscope (Leica TCS SP5). Software: LAS AF version 1.6.3 (Leica Microsystems). Image magnification: 3938x in panel (a) and 1969x in panel (b).

(a)

(b)