Purification and Characterization of Antibodies in Single-Chain Format against the E6 Oncoprotein of Human Papillomavirus Type 16

Figure 5

Internalization of scFvI7nuc protein in SiHa cells. SiHa cells were incubated with DMEM containing rB with (I7nuc) or without (rB) the scFvI7nuc. After 6 hours of treatment, the internalized antibody was visualized using mouse anti-V5 tag mAb followed by secondary antibody conjugated with fluorescein. Microscopy images (a) and immunofluorescence images (b) showing the scFvI7nuc internalization into SiHa cells. Images were captured using FLoid Cell Imaging Station microscope (Thermo Fisher Scientific, USA). Magnification: 600x.

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