(a)
(b)
(c)
Figure 3: Coadministration of the AGN extract with doxorubicin enhanced doxorubicin-induced apoptosis in HeLa cells. (a) The cells were cotreated with the indicated concentrations of doxorubicin and the AGN extract for 24 h, and cell viability was measured using the MTT assay. The statistical significance of the differences, as calculated by Student’s t-test, was determined with p < 0.01. (b) The cells were cotreated with the indicated concentrations of doxorubicin and the AGN extract for 24 h and subjected to immunoblot analyses using specific antibodies as indicated. (c) The cells were cotreated with 1 μM doxorubicin and 1 μg/ml AGN extract for 16 h and apoptosis was analyzed by costaining with annexin V-FITC and Hoechst probes.