Hydroxysafflor Yellow A Promoted Bone Mineralization and Inhibited Bone Resorption Which Reversed Glucocorticoids-Induced Osteoporosis

Figure 3

Effect of HYA on the expression of related genes in osteoporotic zebrafish larvae (n = 15). mRNA levels of the AKP, type I collagen, Runx2, OPG, TRACP, and OCN genes were determined by quantitative real-time PCR. Note: CON, blank E₃ medium; DMSO, blank E₃ medium + 0.5% DMSO; PNSL, blank E₃ medium + 0.5% DMSO + 25 μM prednisolone; DE, 15 μM disodium ethydronate; DE + G, 15 μM disodium ethydronate + PNSL; 0.1, 0.1 μM HYA; 0.1 + G, 0.1 μM HYA + PNSL; 1.0, 1.0 μM HYA; 1.0 + G, 1.0 μM HYA + PNSL; 10.0, 10.0 μM HYA; 10.0 + G, 10.0 μM HYA + PNSL. with CON, p < 0.01. with CON, p < 0.01. with PNSL, p < 0.05. with PNSL, p < 0.01.