Effects of Astaxanthin on Miniature Pig Sperm Cryopreservation

<div>Flow cytometric analysis of sperm labeled with PI/H<sub>2</sub>DCFDA fluorescence in control and AXT treated groups. V1 and V2 regions represent viable sperm with a low intracellular H<sub>2</sub>O<sub>2</sub> radical, and V3 region represents viable sperm with a high intracellular H<sub>2</sub>O<sub>2</sub> radical, and the upper quadrant Dead region represents dead sperm. PI/H<sub>2</sub>DCFDA fluorescence: measure H<sub>2</sub>O<sub>2</sub> of sperm. PI: FL1 Log; H<sub>2</sub>DCFDA: FL3 Log.</div>

BioMed Research International

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Figure 2

Figure 2: Effects of Astaxanthin on Miniature Pig Sperm Cryopreservation 