Aminophylline and Ephedrine, but Not Flumazenil, Inhibit the Activity of the Excitatory Amino Acid Transporter 3 Expressed in Xenopus Oocytes and Reverse the Increased Activity by Propofol

Figure 7

Effects of PI3K inhibition on excitatory amino acid transporter type 3 (EAAT3) activity in the presence or absence of 95.1 μM aminophylline (a) and 1.19 μM ephedrine (B). Whereas preincubation of oocytes with the PI3K inhibitor, wortmannin (1 μM for 1 h), significantly reduced basal EAAT3 activity by 30 μM glutamate, the activity did not differ among wortmannin, aminophylline, or both (a) and among wortmannin, ephedrine, or both (b). PI3K, phosphatidylinositol 3-kinase. Data are means ± SEM, n = 31–46 for each data point. < 0.05 compared with the control.

(a)

(b)