Establishment of a Gene Detection System for Hotspot Mutations of Hearing Loss

Figure 1

Typical genotyping results of the standard plasmids. The graphic information containing values was automatically produced by the software. Melting curves and corresponding genotypes of the nine mutations and wild-type plasmid DNA templates are given according to the four detection channels, shown by the corresponding fluorophores. Tube-A and Tube-B represent the two reactions, respectively.