Establishment of a Gene Detection System for Hotspot Mutations of Hearing Loss

Figure 2

The rs111033313 site detection sensitivity assay result. The concentration of plasmid DNA was 1 × 10¹⁰ to1 × 10¹ copies per reaction. (a) Because of the difference in the initial template concentration, the Ct value increased consistently as the template concentration was reduced. (b) The fluorescence intensity of detection assay results is almost the same and did not consistently change with the initial template concentration. It was suggested that the low concentration sample could still yield an ideal detection result.

(a)

(b)