Establishment of a Gene Detection System for Hotspot Mutations of Hearing Loss

Table 1

Primer sequences.

Primer name	Primer sequences (5′→3′)
Univer Primer-F	GTGGCAGGGCGCTACGAACAAT
Univer Primer-R	CATGTGAGATTGGATCTTGCTGGGC
rs74315319-F	GTGGCAGGGCGCTACGAACAATTCTTCCTCTACCTGCTGCAC
rs74315319-R	CATGTGAGATTGGATCTTGCTGGGCTGGCAGATGAGGTAGCAGAG
rs111033313-F	GTGGCAGGGCGCTACGAACAATAGACACAAAATCCCAGTCCCT
rs111033313-R	CATGTGAGATTGGATCTTGCTGGGCAGAGGAACACCACACTCACC
rs121908362-F	GTGGCAGGGCGCTACGAACAATGAGCAATGCGGGTTCTTTGA
rs121908362-R	CATGTGAGATTGGATCTTGCTGGGCCTAGACTTGTGTAATGTTTGCCA
rs26760661-F	GTGGCAGGGCGCTACGAACAATGAGTAGAGTGCTTAGTTGAACAGGG
rs26760661-R	CATGTGAGATTGGATCTTGCTGGGCAAGTGTAAGTTGGGTGCTTTGTG
rs80338939-F	GTGGCAGGGCGCTACGAACAATTCTCCCTGTTCTGTCCTAGC
rs80338939-R	CATGTGAGATTGGATCTTGCTGGGCTGAGCCAGATCTTTCCAATGC
Tri-sites-F	CATGTGAGATTGGATCTTGCTGGGCAAAGGAGGTGTGGGGAGATG
Tri-sites-R	GTGGCAGGGCGCTACGAACAATATGACCCGGAAGAAGATGCT