Manipulation of Plant Growth Regulators on Phytochemical Constituents and DNA Protection Potential of the Medicinal Plant Arnebia benthamii

Table 2

Effect of different plant growth regulators on shoot multiplication from in vitro raised shoot tips of A. benthamii on MS ×1/2 medium.

BAP (µM)	TDZ; (µM)	IAA (µM)	NAA; (µM)	Shoot number	Shoot length; (cm)	Callusing; (%)	Number of days for minimum shoot formation	Minimum shooting response (%)
-	-	-	-	-	-		-	-
1.5		1.0				+++	55	80
2.5		2.0	-			+	53	80
3.5		3.0	-			+	44	75
4.5		4.0	-			+	40	70
5.5		5.0	-			+	50	80
6.5		6.0	-			+++	-	-
3.5			3.0			+++	50	90
4.5			4.0			++	45	90
5.5			5.0			++	45	85
6.5			6.0			++	35	85
7.5			7.0			++	30	80
8.5			8.0			++	46	80
9.5			9.0			+++	52	90
10.5			10.0			+++	-	-
	1.0	0.5				+	40	90
	2.0	1.0				++	37	100
	3.0	1.5				+	28	100
	4.0	2.0				+	27	100
	5.0	2.5				+	35	90
	6.0	3.0				+	37	90
	7.0	3.5				+	40	90
	8.0	4.0				++	40	90
	3.0		1.5			++	44	80
	4.0		2.0			++	35	80
	5.0		2.5			+	30	90
	6.0		3.0			++	30	95
	7.0	-	3.5			++	38	85

Note. Data scored after 12 weeks of culture period. Data represented as mean ± SD ; + = low intensity callus formation; ++ = moderate intensity callus formation; +++ = high intensity callus formation. Data was statically analyzed using Duncan multiple range test by SPSS 17.0 software. The values followed by different superscripts are statically significant with each other at . TDZ: thidiazuron; IAA: indole 3-acetic acid; Kn: kinetin; BAP: 6-benzylamino purine; IBA: indole 3-butyric acid.