Manipulation of Plant Growth Regulators on Phytochemical Constituents and DNA Protection Potential of the Medicinal Plant Arnebia benthamii

Table 3

Effect of different concentrations of auxins (IBA/IAA) with TDZ on root regeneration of Arnebia benthamii from multiple shoots.

TDZ (µM)	IBA (µM)	IAA (µM)	Root number	Callusing (%)	Rooting response (%)
1.0	0.5	-	-		-
2.0	1.0	-	-		-
3.0	1.5	-		++	70
4.0	2.0	-		+++	80
5.0	2.5	-		++	80
6.0	3.0	-		++	85
7.0	3.5	-		++	85
8.0	4.0	-		+	65
1.0	-	0.5		++	80
2.0	-	1.0		+++	80
3.0	-	1.5		-	85
4.0	-	2.0		-	70
5.0	-	2.5		-	80
6.0	-	3.0		+	70
7.0	-	3.5		+	65
8.0	-	4.0		+++	75

Data scored after 12 weeks of culture period. Data represented as mean ± SD ; + = low intensity callus formation; ++ = moderate intensity callus formation; +++ = high intensity callus formation. Data was statically analyzed using Duncan multiple range test by SPSS 17.0 software. The values followed by different superscripts are statically significant with each other at . TDZ: thidiazuron; IAA: indol 3-acetic acid; IBA: indole 3-butyric acid.