Cyclin G2 Suppresses Glomerulosclerosis by Regulating Canonical Wnt Signalling

Figure 1

Cyclin G2 inhibits the expression of glomerulosclerosis-related proteins and canonical Wnt signalling in HMC cells. (a) Representative western blots and (b) densitometry for FN and collagen IV in HMC cells transduced with lentiviral particles harbouring CCNG2 (LV-CCNG2) or GFP (LV-GFP; control group). (c) Representative western blots and (d) densitometry for FN and collagen IV in CCNG2-overexpressing HMC cells exposed to 30 mM D-glucose (HG) or 5.5 mM D-glucose + 24.5 mM L-glucose (LG; control group) for 72 h. (e) Representative western blots and (f) densitometry for β-catenin, p-β-catenin, GSK3β, p-GSK3β, cyclin D1, and MMP7 in HMC transduced with lentiviral particles expressing CCNG2 (LV-CCNG2) or GFP (LV-GFP; control group). (g) Representative western blots and (h) densitometry for β-catenin, GSK3β, cyclin D1, and MMP7 in CCNG2-overexpressing HMC cells exposed to HG or LG for 72 h. Values are presented as the mean ± SD (n = 3); < 0.05, < 0.01, n.s.: not significant.

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