Cyclin G2 Suppresses Glomerulosclerosis by Regulating Canonical Wnt Signalling

Figure 3

Cyclin G2 deficiency increases renal injury in DN mice. (a, b) PAS staining depicting the degree of renal sclerosis in control WT and Ccng2^−/− mice and STZ-induced WT and Ccng2^−/− DN mice (original magnification, 100×, 400×). (c) HE staining shows glomerular structures (original magnification, 100×, 400×). (d) Representative western blots and (e) densitometry for FN and collagen IV in the renal cortex of control WT and STZ-induced WT and Ccng2^−/− DN mice. (f) Representative collagen IV immunohistochemistry for control WT and Ccng2^−/− mice and STZ-induced WT and Ccng2^−/− DN mice (original magnification, 200×). Values are presented as the mean ± SD (n = 3); < 0.05, < 0.01, n.s.: not significant.

(a)

(b)

(c)

(d)

(e)

(f)