Knockdown of BCL6 Inhibited Malignant Phenotype and Enhanced Sensitivity of Glioblastoma Cells to TMZ through AKT Pathway

Figure 1

Expression of BCL6 mRNA and protein in human glioma tissues and glioblastoma cell lines. (a) The relative expression of BCL6 mRNA in 6 nonmalignant brain tissues and 12 glioma tissues was analyzed by RT-PCR. Data were normalized to the mean of mRNA in normal tissues. (b) The relative expression of BCL6 mRNA in glioblastoma cell lines (U87, A172, SNB19, U251, LN229, and LN308) was analyzed by RT-PCR. Data were normalized to the mean of mRNA in normal tissues. (c) The relative expression of BCL6 protein in nonmalignant brain tissues and glioma tissues was analyzed by western blot. Relative expression was calculated with respect to the first normal tissue. (d) The relative expression of BCL6 protein in glioblastoma cell lines by western blot. Relative expression was calculated with respect to the mean of protein in normal tissues. (e) Transfection efficiency of shBCL6 plasmid was confirmed by real-time PCR and western blot U87 and U251 cells. Relative expression was calculated with respect to the control group without treatment ( p<0.01; p<0.05).

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