Colour Vignetting Correction for Microscopy Image Mosaics Used for Quantitative Analyses

Figure 4

Mosaics of two partially saturated images. The saturated pixels in the overlapping region of the first image are not saturated in the second one. The details reported in the red boxes show a region where the two stitched images do not overlap, while the details in the blue boxes show an overlapping region. (a) Mosaic obtained by stitching the images without performing the vignetting correction. (b) Mosaic obtained through vignetting correcting the images according to (2). Discontinuities generated by the presence of saturated pixels are visible in the two magnified details (highlighted also from arrowheads). (c) Mosaic obtained by vignetting correcting the images according to the OUSPC approach explained in Section 2.2. The discontinuities, previously shown in the blue boxes, disappeared because in correspondence of that overlapping part there are unsaturated pixels available in the other image. Instead, the discontinuities highlighted in the red boxes (i.e., where the two stitched images do not overlap) are still visible.

(a)

(b)

(c)