Colour Vignetting Correction for Microscopy Image Mosaics Used for Quantitative Analyses

Figure 5

Ghost effect due to blending weighting. (a) Mosaic of two partially overlapping images representing a monolayer culture of living cells. Corpuscles and debris floating in the culture medium generate blur objects that look like shadings, when blending is used. In red colour, a magnification detail showing a corpuscle artificially duplicated as a side effect of blending. (b, c) Same detail reported in a red squared, but coming from the two original images composing the mosaic. The corpuscle in motion is present once only for each original image, as shown by the green and yellow arrowheads.

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