Colour Vignetting Correction for Microscopy Image Mosaics Used for Quantitative Analyses

Figure 6

Mosaics of fully saturated images. In the overlapping parts, all the stitched images show pixels saturating in the red channel. (a) Mosaics obtained by stitching four partially overlapped images without performing the vignetting correction. (b) Mosaic obtained by vignetting correcting the images according to Eq. (2). Seams in the stitching zones are still visible (highlighted also from arrowheads). There are no differences with the mosaic built according to the OUSPC approach explained in Section 2.2, because in the overlapping parts all the images of this set show saturated pixels after flat-field correction. (c) Mosaic obtained without performing the flat-field correction, but using the blending approach described in Section 2.3. No seams are visible.

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(b)

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