Inflammatory Human Umbilical Cord-Derived Mesenchymal Stem Cells Promote Stem Cell-Like Characteristics of Cancer Cells in an IL-1β-Dependent Manner

Figure 4

Cytokines derived from cocultured UC-MSCs enhance stem cell-like characteristics in cancer cells. (a) Detection of increased cytokines in 231-MSC coculture compared to MDA-MB-231 cell culture and IGROV1-MSC coculture compared to IGROV1 cell culture by human cytokine array. (b) Relative expression levels of CCL2, CXCL1, and IL-8 in MDA-MB-231 cells and UC-MSCs in 231-MSC coculture and relative expression of CXCL1 and IL-8 in IGROV1 cells and UC-MSCs in IGROV1-MSC coculture. . (c) Relative expression levels of CCL2, CXCL1, IL-8, and IL-6 in UC-MSCs cocultured with MDA-MB-231 cells compared to UC-MSCs cultured alone. . (d) Effects of CCL2 and CXCL1 on pluripotency markers Sox2, Oct4, and KLF4 expression in MDA-MB-231 cells. (e) Effects of CCL2 and CXCL1 on migration ability of MDA-MB-231 cells by Transwell migration assay. , .

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