EGF-Mediated Overexpression of Myc Attenuates miR-26b by Recruiting HDAC3 to Induce Epithelial-Mesenchymal Transition of Lens Epithelial Cells

Figure 5

EGF regulates miR-26b expression in primary HLECs. ((a) and (b)) miR-26b expression in primary HLECs was measured by qRT-PCR. EGF stimulation inhibited the expression of miR-26b in primary HLECs. (a) The primary HLECs were incubated with the indicated concentration of EGF for 48 hours. (b) The primary HLECs were incubated with 10 ng/ml EGF for the indicated time. EGF treatment decreased the expression of miR-26b in a dose- and time-dependent manner. (c) Primary HLECs were preincubated with EGFR-specific siRNAs, Myc-specific siRNAs, or EZH2-specific siRNAs for 24 hours before incubation with 10 ng/ml EGF for 48 hours. miR-26b expression in primary HLECs was measured by qRT-PCR. compared with group without EGF.

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