EGF-Mediated Overexpression of Myc Attenuates miR-26b by Recruiting HDAC3 to Induce Epithelial-Mesenchymal Transition of Lens Epithelial Cells

Figure 6

miR-26b expression suppressed by EGF is controlled by HDAC3 in primary HLECs. (a) Suberoylanilide hydroxamic acid (10 μmol/L), apicidin (3 μmol/L), OSU42 (2.5 μmol/L), or TSA (0.4 μmol/L) significantly attenuated the expression of miR-26b in primary HLECs suppressed by EGF. miR-26b expression in primary HLECs was measured by qRT-PCR. (b) Primary HLECs were preincubated with HDAC1~11-specific siRNAs for 24 hours before incubation with 10 ng/ml EGF for 48 hours. miR-26b expression in primary HLECs was measured by qRT-PCR. compared with normal (a). compared with EGF (b).

(a)

(b)