EGF-Mediated Overexpression of Myc Attenuates miR-26b by Recruiting HDAC3 to Induce Epithelial-Mesenchymal Transition of Lens Epithelial Cells

Figure 7

miR-26b directly controls EZH2 expression in HLECs. (a) EZH2 and β-actin were detected in primary HLECs after transfection with miR-26b mimics or miR-26b mimics control for 6 h by Western blot analysis. (b) Western blotting for EZH2 and β-actin in primary HLECs after transfection with anti-miR-26b or anti-miR-control for 6 h. (c) The luciferase reporter assays in the SRA01/04 cell line were measured. The miR-26b mimics or miR-26b mimics controls were cotransfected into plasmids with wild-type or mutant EZH2 3′-UTRs. compared with Mock ((a) and (b)). compared with miR-con mimics (c).

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