BioMed Research International

Research Article

EGF-Mediated Overexpression of Myc Attenuates miR-26b by Recruiting HDAC3 to Induce Epithelial-Mesenchymal Transition of Lens Epithelial Cells

Table 1

Primers used for qRT-PCR and cloning.
PrimersSequence
SenseAntisense
GAPDH5′-AGGTCGGTGTGAACGGATTTG-3′5′-TGTAGACCATGTAGTTGAGGTCA-3′
U65′-CTCGCTTCGGCAGCACA-3′5′-AACGCTTCACGAATTTGCGT-3′
miR-26b5′-CTCAAGGGCTTGTGCTGACT-3′5′-ACCTCAGCCTAGTGCAGGAA-3′
Fibronectin5′-TCTGTGCCTCCTATCTATGTGC-3′5′-GAGGGACCACGACAACTCTTC-3′
α-SMA5′-CCGACCGAATGCAGAAGGA-3′5′-ACAGAGTATTTGCGCTCCGAA-3′
Myc5′-TCAGAGAAGCTGGCCTCCTA-3′5′-TCGTTGGAGGAGAGCAGAGA-3′
EZH25′-CCAACATTGGAGTGATTCAG-3′5′-TCATCAGATGATTTAGCCCA-3′
HDAC15′-GCGAGCAAGATGGCGCAGACT-3′5′-GTGAGGCTTCATTGGGTGCCCT-3′
HDAC25′-TGAAGCCAAACTTCCTCAAACA-3′5′-CGGAGGACAAGAGGACAGATG-3′
HDAC35′-CACATCGTCATCTCGATTTCCT-3′5′-GGCATGGCTCTCTGAAACCTTA-3′
HDAC45′-GAGTACTGCCGAGCAGTCTGAA-3′5′-CACTTTAATTGGGTCTGGAGGC-3′
HDAC55′-TTCTTCAACTCCGTAGCC-3′5′-TCCCATTGTCGTAGCG-3′
HDAC65′-TGTGGCTGCCCGCTATGCAC-3′5′-GGGGCCAGAACCGACCATGC-3′
HDAC75′-ACCCAACCTCAATGCC-3′5′-GATGCCAACGGAAAGG-3′
HDAC85′-CCAGCCACAGAAGGGATA-3′5′-TTCCGTCGCAATCGTAAT-3′
HDAC95′-GTCCCTGCCCAATATCAC-3′5′-GCTGTTCGGTTTGCCCTC-3′
HDAC105′-CCGGCAGAGGGCGTGTTGAG-3′5′-CAAGGCAGCTGTCAGGCGCT-3′
HDAC115′-ACAACCGCCACATCTAC-3′5′-AGGGACCTCCTCACATT-3′
EZH2-3′UTR-WT-luc5′-TATCTAGACATCTGCTACCTCCTCCC-3′5′-ATGCGGCCGCGATTCAACAAGGACAA-3′
EZH2-3′UTR-MT-luc5′-TATCTAGACATCTGCTACCTCCTCCC-3′5′- CTGCTACCTCCTCCC-3′