Modulation of Molecular Biomarker Expression in Response to Chemotherapy in Invasive Ductal Carcinoma

Table 2

Sequences of biomarker primers used in this study (F, forward primer; R, reverse primer).

Gene	Marker description	Ref.
CK19	General marker upregulated in epithelial tumor; F 5′-ATGAAAGCTGCCTTGGAAGA-3′; R 5′-TGATTCTGCCGCTCACTATCAG-3′	[9]
hMAM	Secretory epithelial protein overexpressed in breast cancer; F 5′-CCATGAAGTTGCTGATGGTC-3′; R 5′-TCAGAGTTTCATCCGTTTGG-3′	[10]
HER2/neu	Receptor tyrosine kinase associated with signal transduction; F 5′-GGATATCCAGGAGGTGCAGGGTAC-3′; R 5′-CCTGTGAGGCTTCGAAGCTGCAGCT-3′	[11]
MUC1	Membrane mucin present in epithelia, participating in cellular signaling; F 5′-CGTCGTGGACATTGATGGTACC-3′; R 5′-GGTACCTCCTCTCACCTCCTCCAA-3′	[12]
Myc	Transcription factor involved in apoptosis and cell proliferation; F 5′-CAGCTGCTTAGACGCTGGATTT-3′; R 5′-ACCGAGTCGTAGTCGAGGTCAT-3′	[13]
Ki-67	Marker for proliferating cells; F 5′-ATCGTCCCAGGTGGAAGAGTT-3′; R 5′-ATAGTAACCAGGCGTCTCGTGG-3′	[14]
ErbB2	Oncogene code for epidermal growth factor receptor (GFR); F 5′-CTGGTGACACAGCTTATGCCCT-3′; R 5′-ATCCCCTTGGCAATCTGCA-3′	[12]
ERα/β	Estrogen receptor alpha and beta; F 5′-TGCTTCAGGCTACCATTATGGAGTCTG-3′; R 5′-GTCAGGGACAAGGCCAGGCTG-3′; F 5′-TTTAAAGAAGCATTCAAGGACATAATG-3′; R 5′-GAAGTGTGGCTCCCGGAGAGAGAG-3′	[14]
CEA	Glycoprotein involved in cell adhesion; F 5′-TCTGGAACTTCTCCTGGTCTCTCAGCTGG-3′; R 5′-TGTAGCTGTTGCAAATGCTTTAAGGAAGAAGC-3′; F 5′-GGGCCACTGTCGGCATCATGATTGG-3′; R 5′-TGTAGCTGTTGCAAATGCTTTAAGGAAGAAGC-3′	[15]
GAPDH	Housekeeping gene; used to normalize the expression; F 5′-GGTCGGAGTCAACGGATTTG-3′; R 5′-ATGAGCCCCAGCCTTCTCCAT-3′	[9]