Time-Dependent Effects of POT1 Knockdown on Proliferation, Tumorigenicity, and HDACi Response of SK-OV3 Ovarian Cancer Cells

Figure 2

POT1 mRNA expression as determined by qRT-PCR (a) and POT1 protein expression as determined by western blot analysis (b) in shRNA-infected human ovarian cancer SK-OV3 cells. POT1-KD significantly decreased cell proliferation (c) and colony formation (d) in the early POT1-KD SK-OV3 cells. “Negative control” indicates negative control SK-OV3 cells that express a nonspecific shRNA, and “POT1-KD” represents POT1-knockdown SK-OV3 cells that express POT1-specific shRNA. (c) shows the cPDs of the SK-OV3 cells. The cPDs of the negative control cells are depicted with black dots and lines, while the cPDs of the immediate effect POT1-KD cells are depicted with black squares and lines. (d) shows images from the soft agar colony formation assay that used SK-OV3 cells. The dark dots represent the colonies. Images of the negative control cells and the immediate effect POT1-KD cells were captured via digital phase-contrast microscopy at 4x magnification; scale bar: 1000 μm. , compared with negative control cells.

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