Protective Role of Coxsackie-Adenovirus Receptor in the Pathogenesis of Inflammatory Bowel Diseases

Figure 4

Overexpression of CAR attenuates on TNF-α induced cell permeabilization in human colon cell lines. The effect of CAR overexpression on NCM460 and SW480 monolayer barrier function. (a & b) FITC-Dextran cell permeability assay evaluation of human colon cell permeability upon CAR overexpression. Monolayers of NCM460 (a) and SW480 (b) cells were plated on upper-chamber of 12-well plates, after CAR overexpression and TNF-α induction, FITC-Dextran reagent was added to the upper-chamber, and, 30 min later, the concentration of FITC-Dextran in the lower compartment of 12-well plate was measured to determine the permeability of cell monolayers. (c & d) Effect of CAR on TEER of human colon cell lines. NCM460 (c) and SW480 (d) cells were transfected with CAR overexpression plasmid or control plasmid and treated with control or TNF-α, and TEER was measured to determine the barrier integrity of cells. All data represent at least 3 independent experiments. , , and ; data represent mean ± s.e.m.

(a)

(b)

(c)

(d)