MicroRNA Expression Profiling in Psoriatic Arthritis

Figure 5

Validation of differentially expressed miRNAs by real-time PCR. (a) Real-time PCR for the indicated miRNAs were performed in healthy controls (Healthy), PsA active, and nonactive samples included in the microarray and in 7 patients affected by RA. (b) Real-time PCR for miR-126-3p in an expanded cohort of healthy (15 patients), PsA active (11 patients), and PsA nonactive (12 patients) groups. Values are calculated with method. miR-16-5p and miR-26a-5p were used as endogenous controls for miRNA expression (see Methods). Histograms indicate mean values; bars indicate standard deviation (SD). ; ; (Mann–Whitney rank sum test). (c) Real-time PCR expression of miR-126-3p in Jurkat cells after 48 h from transfection with miRNA mimics. miR-16-5p and miR-26a-5p were used as endogenous controls. (d) Identification of novel miR-126-3p targets. Real-time PCR expression of the indicated transcripts in mimic miR-126-3p-transfected and control cells. Beta-Actin was used as endogenous control. Values were calculated with method as fold change with respect to mimic negative control-transfected samples. All the histograms indicate mean values of at least three independent experiments; bars indicate standard deviation (SD). ; ; (Student’s -test).

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