Comparative Proteomic Study of the Antiproliferative Activity of Frog Host-Defence Peptide Caerin 1.9 and Its Additive Effect with Caerin 1.1 on TC-1 Cells Transformed with HPV16 E6 and E7

Figure 2

The cell apoptosis assay of TC-1 cells exposed to different concentrations of caerin 1.1, caerin 1.9, and P3 treatments and untreated TC-1 cells for 24 h by flow cytometric analysis after double-staining with Annexin/PI. (a), (b), and (c) show representative graphs obtained in the presence of caerin 1.1, caerin 1.9, and P3 at 10, 5, and 1 μg/mL, respectively; (d) untreated TC-1 cells; (e) percentage of apoptotic (Q2 + Q3) and necrotic (Q1) cells detected in different treatments and control cells after 24 h. The bar graph represents the mean ± s.d. for independent experiments for pooled early and late apoptotic cells. Statistical analysis was performed by the two-tailed t-test. , , and versus control.

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