Comparative Proteomic Study of the Antiproliferative Activity of Frog Host-Defence Peptide Caerin 1.9 and Its Additive Effect with Caerin 1.1 on TC-1 Cells Transformed with HPV16 E6 and E7

Figure 3

Overall workflow for characterisation of antiproliferation activity of peptide caerin 1.1 and caerin 1.9 against cervical cancer TC-1 cells, using bioassays in conjunction with quantitative proteomics. (a) Treated and untreated cell and supernatant proteins/peptides were extracted and labelled with iTRAQ, followed by identification and quantification with high-accuracy nano-LC Triple TOF-MS. (b) The comparison of proteins identified from cells (three replicates). (c) The comparison of protein identified from supernatant (three replicates used in untreated or treated conditions).