Peroxisome Proliferator-Activated Receptor- Prevents Cholesterol Gallstone Formation in C57bl Mice by Regulating Bile Acid Synthesis and Enterohepatic Circulation

Figure 5

Effects of activation of PPAR on expressions of genes involved in BA synthesis and cholesterol transport in liver tissues of mice fed on LGD. (a, b) mRNA expressions of hepatic liver X receptor α, peroxisome proliferator-activated receptor-γ coactivator α (PGC-1α), and hepatic cholesterol and phospholipid transporters (ATP-binding cassette transporter G5 (ABCG5), ABCG8, ABCA1, ABCG1, ABCB4). (c) Key enzymes involved in BA synthesis (cytochrome P450 enzyme 7A1 (CYP7A1), CYP8B1, CYP27A1), as determined by real-time RT-PCR. (d) Western blotting results of hepatic CYP7A1. (e) mRNA expressions of intestinal cholesterol transporters (Niemann-Pick C 1-like 1 (NPC1L1), ABCG5, and ABCG8). (f) Fecal cholesterol content was detected and measured with a fully automatic analyzer. Data are presented as the means ± SEM (n=7/group). P<0.05; P<0.01.

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