Detection of Epithelial-Mesenchymal Transition Status of Circulating Tumor Cells in Patients with Esophageal Squamous Carcinoma

Table 1

Capture probe sequences for the biomarkers.

Biomarker	Sequences (5’-3’)
CD45	TCGCAATTCTTATGCGACTC
	TGTCATGGAGACAGTCATGT; GTATTTCCAGCTTCAACTTC; CCATCAATATAGCTGGCATT; TTGTGCAGCAATGTATTTCC; TACTTGAACCATCAGGCATC
EpCAM	TGGTGCTCGTTGATGAGTCA
	AGCCAGCTTTGAGCAAATGA; AAAGCCCATCATTGTTCTGG; CTCTCATCGCAGTCAGGATC; TCCTTGTCTGTTCTTCTGAC; CTCAGAGCAGGTTATTTCAG
CK8	CGTACCTTGTCTATGAAGGA
	ACTTGGTCTCCAGCATCTTG; CCTAAGGTTGTTGATGTAGC; CTGAGGAAGTTGATCTCGTC; CAGATGTGTCCGAGATCTGG; TGACCTCAGCAATGATGCTG
CK18	AGAAAGGACAGGACTCAGGC
	GAGTGGTGAAGCTCATGCTG; TCAGGTCCTCGATGATCTTG; CAATCTGCAGAACGATGCGG; AAGTCATCAGCAGCAAGACG; CTGCAGTCGTGTGATATTGG
CK19	CTGTAGGAAGTCATGGCGAG
	AAGTCATCTGCAGCCAGACG; CTGTTCCGTCTCAAACTTGG; TTCTTCTTCAGGTAGGCCAG; CTCAGCGTACTGATTTCCTC; GTGAACCAGGCTTCAGCATC
Vimentin	GAGCGAGAGTGGCAGAGGAC
	CTTTGTCGTTGGTTAGCTGG; CATATTGCTGACGTACGTCA; GAGCGCCCCTAAGTTTTTAA; AAGATTGCAGGGTGTTTTCG; GGCCAATAGTGTCTTGGTAG
Twist	ACAATGACATCTAGGTCTCC
	CTGGTAGAGGAAGTCGATGT; CAACTGTTCAGACTTCTATC; CCTCTTGAGAATGCATGCAT; TTTCAGTGGCTGATTGGCAC; TTACCATGGGTCCTCAATAA