BioMed Research International

Research Article

-Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum

Table 1

Primers for -shift method based on five SNPs.
PrimerNucleotide sequence (5′–3′)Product length (bp)
ITS71TF(Aca)GCGGCGCTATGTGCAGCAAGAGT 
ITS71CF(Ace)GCGGGCAGGGCGGGCTATGTGCAGCAAGAGC113
ITS71RACAAGCAGTAAGGCGGCATTCA 
ITS197GF(Aca)GCGGCGTGAGCATTAGGCTAACGCCTG 
ITS197AF(Ace)GCGGGCAGGGCGGGTGAGCATTAGGCTAACGCCTA114
ITS197RACGATTCTGCAAACATTAAACGTAAAAAGT 
ITS296TF(Ace)GCGGCGTTTGCAGAATCGTGACTTT 
ITS296GF(Aca)GCGGGCAGGGCGGGTTTGCAGAATCGTGACTTG127
ITS296RTTCACCACTCTAAGCGTCT 
ITS26AF(Aca)GCGGCGGAAGGTTGGGAGTATCA 
ITS26GF(Ace)GCGGGCAGGGCGGGGAAGGTTGGGAGTATCG121
ITS26RGTCTAAAGCTCAGCGAAAC 
ITS48AF(Aca)GCGGCGCCGTTACAGCCCTACGTA 
ITS48GF(Ace)GCGGGCAGGGCGGGCCGTTACAGCCCTACGTG116
ITS48RATGCAATGCTCATCAAGTC 
The GC tails are bold and underlined. Primers at ITS26 and ITS48 failed to distinguish between A. ceylanicum and A. caninum.