Simvastatin Impairs the Inflammatory and Repair Phases of the Postinjury Skeletal Muscle Regeneration

Figure 2

Effect of SIM on the inflammatory phase of the postinjury skeletal muscle regeneration. To assess this effect, the presence and degree of extravasation (a), the duration and degree of necrosis (b), and the presence and intensity of inflammation (c) were evaluated in the BPVC-injured muscles of nontreated (control) and SIM-treated animals. The results are expressed as the mean scores (±SDs) from six sites of muscle injury derived from three animals (two independent muscle injuries per single animal) per group per day. The P values (P < 0.05, P < 0.01, and P < 0.001) indicate the significance of the differences between groups at the same time point. Representative H&E-stained sections of postinjury myofibre regeneration sites. Day 1: extensive necrosis and marked inflammation were observed in the control and SIM-treated groups. Day 7: mild inflammation, moderately numerous myotubes and numerous young myofibres were observed in the control group. Extensive necrosis and extravasation, moderate inflammation, and moderately numerous myotubes were detected in the SIM-treated group. Day 10: not numerous myotubes and numerous young myofibres were found in the control group, whereas focal necrosis, mild inflammation, not numerous myotubes, and moderately numerous young myofibres were detected in the SIM-treated group (d).

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