Simvastatin Impairs the Inflammatory and Repair Phases of the Postinjury Skeletal Muscle Regeneration

Table 1

Summary of the immunohistochemical methodology.

Primary antibody	Clone	Dilution	Antigen retrieval	Visualization system
Anti–MyoD1	5.8A	1:50	2x3 min†,; Tris-EDTA buffer pH 9.03 min	EnVision + System-HRP, Mouse (DAB)
Anti–Desmin	D33	1:50	2x3 min†,; Tris-EDTA buffer pH 9.03 min	EnVision + System-HRP, Mouse (DAB)

DAKO, Glostrup, Denmark; † antigen retrieval was conducted in a microwave oven at 650 W.