Research Article
Simvastatin Impairs the Inflammatory and Repair Phases of the Postinjury Skeletal Muscle Regeneration
Table 2
Scoring system used to evaluate the inflammatory and repair phases of myofibre regeneration.
| | Category | Evaluation criterion | Points assigned per criterion |
| | Extravasation | Absent | 0 | | Focal | 1 | | Extensive | 2 |
| | Necrosis (myofibre hypercontraction, hyalinization and fragmentation of the cytoplasm, loss of striations) | Absent | 0 | | Single fibres | 1 | | Focal | 2 | | Extensive | 3 |
| | Inflammation (all inflammatory cells, i.e., neutrophils macrophages, lymphocytes, eosinophils) | Absent | 0 | | Minimal: ≤5 inflammatory cells per HPF (40x) | 1 | | Mild: 6-10 inflammatory cells per HPF (40x) | 2 | | Moderate: 11-20 inflammatory cells per HPF (40x) | 3 | | Marked: >20 inflammatory cells per HPF (40x) | 4 |
| | Myogenic precursor cells (MPCs) | Absent | 0 | | Not numerous: ≤3 MPCs per HPF (40x) | 1 | | Moderately numerous: 4-6 MPCs per HPF (40x) | 2 | | Numerous: 7-12 MPCs per HPF (40x) | 3 | | Markedly numerous: >12 MPCs per HPF (40x) | 4 |
| | Myotubes | Absent | 0 | | Not numerous: <3 myotubes per HPF (40x) | 1 | | Moderately numerous: 3-5 myotubes per HPF (40x) | 2 | | Numerous: >5 myotubes per HPF (40x) | 3 |
| | Young myofibres | Absent | 0 | | Not numerous: <3 young myofibres per HPF (40x) | 1 | | Moderately numerous: 3-5 young myofibres per HPF (40x) | 2 | | Numerous: >5 young myofibres per HPF (40x) | 3 |
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