Improvement Activity of 1-Deoxynojirimycin in the Growth of Dairy Goat Primary Mammary Epithelial Cell through Upregulating LEF-1 Expression

Figure 1

Effect of DNJ on the growth of dairy goat primary mammary epithelial cells. (a) Effect of a series of DNJ solutions with different concentration on the primary mammary epithelial cell growth. Dairy goat primary mammary epithelial cells were seeded into a 6-well plate at the density of 3 × 10⁵/cm². Then the cells were treated with a series of DNJ solutions with different concentration for 72 h and were observed under a microscope (100x). (b) LEF-1 mRNA levels in the primary mammary epithelial cells treated with a series of DNJ solutions with different concentration were detected by using real-time qPCR. (c) Relative growth of the primary mammary epithelial cell in each group of (a). (d) The protein levels of LEF-1 in mouse mammary glands were detected using western blotting. Data were expressed as mean ± SEM, compared with control (DMSO); indicated ; indicated .

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