2³ Full Factorial Model for Particle Size Optimization of Methotrexate Loaded Chitosan Nanocarriers: A Design of Experiments (DoE) Approach

Table 2

Experimental design of 2³ full factorial design with particle size.

Run	Chitosan	TPP	MTX	Particle size	PDI
	(mg/ml)	(mg/ml)	(mg)	(nm)
1	1	0.8	30	147	0.465
2	1	0.8	60	168	0.682
3	1	1	30	159	0.653
4	1	1	60	182	0.504
5	1.5	0.9	45	203	0.497
6	1.5	0.9	45	205	0.468
7	1.5	0.9	45	199	0.504
8	2	0.8	30	228	0.419
9	2	0.8	60	256	0.578
10	2	1	30	241	0.423
11	2	1	60	268	0.612

= center points included in the design.