Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells

Figure 1

The expression of FBXO5 was upregulated after osteogenic induction in hPDLSCs. (a) Western blot results revealed that the expression of FBXO5 in hPDLSCs increased at 5, 7, and 14 days after induction. (b) Quantitative analysis of FBXO5 expression in hPDLSCs based on the results of western blotting. (c) The difference between FBXO5 transcript a (NM_001142522.2) and transcript b (NM_012177.4) was located in exons 1 and 2. (d) Comparison of the three-dimensional structure of the two isoforms using SWISS-MODEL analysis. In comparison with isoform b, isoform a had a shorter N-terminus. Real-time RT-PCR results showed that the expression of FBXO5 gene (e), transcript a (f), and transcript b (g) increased at 7 days after osteogenic induction. GAPDH was used as an internal control. Student’s -test was performed to determine statistical significance. Error bars represent the standard deviation (). . .

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