Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells

Figure 3

Knockdown of FBXO5 inhibited the osteogenic differentiation of hPDLSCs. Transduced cells were cultured with normal medium (NM) or osteogenic differentiation medium (OM). After 5 days of induction, alkaline phosphatase (ALP) staining (a) and ALP activity (b) results showed that ALP activity was inhibited in FBXO5sh-treated hPDLSCs. After 2 weeks of induction, alizarin red staining (c) and calcium quantitative analysis (d) results revealed that mineralization was inhibited in FBXO5sh-treated hPDLSCs. After 7 days of induction, western blot results (e) and quantitative analysis (f) revealed a significant decrease in the expression of RUNX2, OSX, and OCN in FBXO5sh group as compared with CONTsh group. GAPDH was used as an internal control. Student’s -test was performed to determine statistical significance. Error bars represent the standard deviation (). . . Scale bars represent 200 μm.

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