Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells

Figure 5

Overexpression of FBXO5 enhanced the osteogenic differentiation of hPDLSCs. Transduced cells were cultured with normal medium (NM) or osteogenic differentiation medium (OM). After 5 days of induction, alkaline phosphatase (ALP) staining (a) and ALP activity (c) results showed that both HA-FBOX5 a and HA-FBXO5 b enhanced ALP activity in hPDLSCs. After 2 weeks of induction, alizarin red staining (b) and calcium quantitative analysis (d) results revealed that both HA-FBOX5 a and HA-FBXO5 b enhanced mineralization in hPDLSCs. After 7 days of induction, western blot results (e) and quantitative analysis (f) showed a significant increase in the expression of RUNX2 and OSX in HA-FBOX5 a and HA-FBXO5 b group as compared to the vector group. The expression of OCN significantly increased in HA-FBOX5 a group compared with the vector group. GAPDH was used as an internal control. Student’s -test was performed to determine statistical significance. Error bars represent the standard deviation (). . . Scale bars represent 200 μm.

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