Rapamycin Inhibits the Growth and Collagen Production of Fibroblasts Derived from Human Urethral Scar Tissue

Figure 2

Rapamycin inhibits the growth of FHUS. FHUS were exposed to six different concentrations of rapamycin (0, 10, 20, 40, 80, or 160 ng/ml) for either 24 or 48 h. The MTT assay was used to identify viable cells, and the cell growth inhibition rate was calculated as (1 – A570 of rapamycin-treated cells/A570 of control group) × 100%. Rapamycin caused a concentration-dependent inhibition of FHUS growth at 24 (a) and 48 (b) h. Data shown as mean ± standard deviation ( cell lines from four patients). versus the control group (0 ng/ml rapamycin).

(a)

(b)