Technetium-99 Conjugated with Methylene Diphosphonate Ameliorates Glucocorticoid Induced Osteoporosis by Inhibiting Osteoclastogenesis

Figure 2

3D structure of cancellous bone from micro-CT (0.4mm thick) and characteristics of trabeculae visualized by H&E staining (magnification: 50×).  ⁹⁹Tc-MDP modulated the density, integrity, and connectivity of trabeculae changed by glucocorticoid (left panel). Staining with hematoxylin and eosin (H&E), sparse, and disordered trabeculae induced by glucocorticoid was improved after the treatment of ⁹⁹Tc-MDP (right panel). (a) Blank group, (b) negative control group, (c) high dose group, (d) medium dose group, (e) low dose group, and (f) positive control group.