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BioMed Research International
Volume 2018, Article ID 7907092, 11 pages
Research Article

Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence of In Vitro Grown and Matured Oocytes

1Department of Biomedical Laboratory Science, Eulji University, Seongnam, Gyeonggi-do, Republic of Korea
2Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Republic of Korea
3Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea
4Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Republic of Korea

Correspondence should be addressed to Jung Ryeol Lee;

Received 18 October 2017; Revised 30 January 2018; Accepted 28 February 2018; Published 4 April 2018

Academic Editor: Weon-Young Son

Copyright © 2018 Jaewang Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In vitro follicle growth (IVFG) is an emerging fertility preservation technique, which can obtain fertilizable oocytes from an in vitro culture system in female. This study aimed to compare efficiency of the most widely used two-dimensional follicle culture methods [with or without oil layer (O+ or O− group)]. Preantral follicles were isolated from mice and randomly assigned. Follicles were cultured for 10 days and cumulus-oocyte complexes harvested 16–18 hours after hCG treatment. Follicle and oocyte growth, hormones in spent medium, meiotic spindle localization, expression of reactive oxygen species (ROS), mitochondrial activity, and gene expression were evaluated. In follicle growth, survival, pseudoantral cavity formation, ovulation, and oocyte maturation were also significantly higher in O+ group than O− group. Hormone production was significantly higher in follicles cultured in O+ than O−. There were no significant differences in mRNA expression related to development. On the other hand, the level of ROS was increased while the mitochondrial activity of in vitro grown matured oocyte was less than in vivo matured oocytes. In conclusion, follicle culture with O+ group appears to be superior to the culture in O− group in terms of follicle growth, development, oocyte growth, maturation, and microorganelles in oocyte.