Negative Regulation of PTEN by MicroRNA-221 and Its Association with Drug Resistance and Cellular Senescence in Lung Cancer Cells

Figure 5

miR-221 regulates PTEN/Akt pathway. (a) Expression of miR-221 was examined by PCR in H1299-miR-221 cells and its paired control cells (H1299-Cont). (b) Expression of miR-221 was examined by real-time polymerase chain reaction (PCR) in A549-anti-miR-221 cells and its paired control cells (A549-Cont). (c) Lysates of H1299-miR-221 and its paired control cells (H1299-Cont) were subjected to Western blot analysis using a specific antibody for detection of PTEN and pAkt. (d) Lysates of A549-anti-miR-221 cells and its paired control cells (A549-Cont) were subjected to Western blot analysis using a specific antibody for detection of PTEN and pAkt. Ct values were normalized with control mammalian U6 snRNA (dCt).

(a)

(b)

(c)

(d)