A Novel UDP-Glycosyltransferase of Rhodiola crenulata Converts Tyrosol to Specifically Produce Icariside D2

Table 1

The primers used in this study.

Primer	Sequence(-)	Aim
dF	GGCATCCCGCGGCTNGTNTTYCAYG	Cloning of the core sequence
dR	CGGTCACCAGCTTCTCGTTGWARAAYTGNTC	Cloning of the core sequence
Primer 3′-1	GCCGGACTCGGTCGTGTACGT	3′ RACE
Primer 3′-2	GAGAAGTTCCGAGTCAGGAT	3′ RACE
Primer 5′-1	GACCATTTCTGCACTCCAACCT	5′ RACE
Primer 5′-2	GAACAACGGCCACDTCACCAT	5′ RACE
pF	CGCGGATCCATGGATTCTGATTCACGGCCT	Vector construction
pR	CGCGGTACCCTAGGACAAAGGCACTCTTCT	Vector construction
Primer qF	ACTCATTGCGGATGGAAT	qPCR
Primer qR	CCTTCTCAACCTTCTCCTT	qPCR