Indole-3-Carbinol Induces Apoptosis in Human Osteosarcoma MG-63 and U2OS Cells

Figure 1

Inhibitory effect of I3C on migration of MG-63 and U2OS cells. Wound healing assay of I3C-treated MG-63 (a) and U2OS (b) cells. The number of cells migrating to the wound increased in vehicle-treated MG-63 and U2OS cells, whereas fewer cells migrated to the wound area in I3C-treated cells. This migration was dose-and time-dependent. (c) Invasion of MG-63 and U2OS cells was evaluated by Boyden chamber assay after treatment with different concentrations of I3C. (d) Invading cells were counted and the results were analyzed statistically using Student’s t-test. = p<0.05. (e) Proteolytic activities of MMP-2 and MMP-9 were attenuated by the treatment of I3C in MG-63 and U2OS cells. (f) Activities of MMP-2 and MMP-9 were analyzed statistically using Student’s t-test. , # = p<0.05.

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